April 2024 goals and daily notebook entries.

Goals

Writing and manuscripts

1. Submit molecular underpinnings (Ch.1)
2. A.pul gametogenesis final figures, results sections for histology analysis
3. Marine heatwaves manuscript figures

Sample processing

1. Histological image analysis (abundance and size)
2. Library prep for heatwave WGBS samples (12)

Data analysis

1. Work on DEG analysis for heatwave after mapping sequences to de novo transcriptome
2. Histological image/gonad analyses

Daily log

April 1

• BIO 101 TA 11-12pm
• BIO 101 TA meeting 12-1pm
• Made initial Ch2 histology plots
• Finalized environmental and physiological Ch2 plots
• Finished environmental and physiological methods and results paragraphs for Ch2
• Worked with James and electrician in CBLS wetlab for light work request
• Aquarium duties

April 2

• Putnam writing/journal club meeting 12-1:30pm
• Looking into isoform identification softwares for Ch3 duplicate copy issues
• Finished write up for BUSCO duplicate copy issues
• Hollie meeting
• Concussion diagnosis - need to take time off the next couple days from screen time

April 3

• BIO 101 TA 11-12pm
• Home - concussion rest

April 4

• BIO 101 office hours 9-12pm
• Hollie meeting summary
• Ch2 methods for abundance and size
• Brightspace grading
• Titrations
• Concussion rest

April 5

• Concussion rest
• Ch2 manuscript methods edits

April 6

• Concussion rest
• Ch2 manuscript methods edits

April 8

• Molecular underpinnings abstract edits
• BIO 101 TA and meeting 11-1pm
• Ch2 manuscript methods edits
• Prepped sample and coral shipment labels and supplies

April 9

• Packaged and sent corals and samples to Barott and Burgess labs
• Putnam writing meeting 11-12:30pm
• Updated data for DMG results sections and figures for molecular underpinnings
• Finished molecular underpinnings abstract/introduction edits

April 10

• Updated results and discussion for molec underpinnings
• TA 11-1pm
• BS_SNPer meeting discussion
• Histology analysis

April 11

• Updated results and discussion for molec underpinnings
• Practice defense preperation 11-1:30pm
• Aquarium duties

April 12

• +5 TA tasks
• +5 TA meeting 9:30-10:30am
• TA 11-12pm
• Defense 1:30-2:30pm
• Finished edits to molec underpinnings results and discussion
• Ch3 isoform analysis
• Ch2 metadata exploration and general additive model literature review

April 15

• Added information and updates requested to Pocillopora manuscript
• Ch3 isoform analysis
• Histology analysis
• BIO 101 TA 11-1pm
• SNP-WGBS meeting 3-4pm
• Ch2 research for egg protein and biomass literature review
• Ch2 metadata exploration and general additive model literature review

April 16

• Ch2 research for egg protein and biomass literature review
• Ch3 isoform analysis
• Ch2 metadata exploration and general additive model literature review
• Putnam journal club/writing meeting 11-12:30
• Worked on lab presentation BUSCO
• Hollie meeting 2:30-3:30pm
• Aquarium DMs and cleaning

April 16

• Histological analysis continued
• Wrote hypotheses and restructured introduction for Ch2
• 11-12pm BIO 101 TA
• 12- 1:30pm Putnam lab meeting
• BIO 101 TA test corrections 3-5pm

April 17

• Histological analysis continued
• Wrote hypotheses and restructured introduction for Ch2
• 11-1pm BIO 101 test corrections
• +5 TA weekly work
• Aquarium DMs and cleaning

April 18

• Ch2 writing intro paragraph and hypotheses
• BIO 101 exam corrections 11-1pm
• Off for DC

April 19

• Off for DC

April 22

• Off for DC

April 23

• Histology analysis
• Ch3 analysis and outlier clean up
• Putnam writing 11-12:30pm, writing for Ch2 introduction and hypotheses
• Histology analysis
• Aquarium DMs and cleaning

April 24

• Histology analysis
• Ch3 analysis and outlier clean up
• 11-12pm BIO 101 TA
• Presented at lab meeting for transcriptome 12-1:30pm
• Condensed some A. pulchra decalcified samples (December-March 2022)

April 25

• UNCW visit

April 26

• UNCW visit

April 29

• Molec underpinnings edits from Hollie for introduction
• BIO 101 TA 11-1pm
• Ch2 hypothesis and introduction edits
• Condensed some A. pulchra decalcified samples (April - July 2022)

April 30

• Histology analysis
• 11-12:30 pm Putnam lab meeting
• Aquarium titrations
• BIO 101 TA support celebration