Tracking Total Alkalinty in the CBLS WetLab Tank System

Titrations to Track Total Alkalinity in the CBLS WetLab Tank System

Goal

Conduct titrations to track the total alkalinity of the CBLS WetLab tank system located on the bottom floor of the Center for Biotechnology and Life Sciences at URI. The Putnam Lab conducts various experiments within the tank system which requires stable tank conditions.

CBLS Wetlab Map

Tank Identifications

Blue Tank = Water Table = BT

Quarantine Tank = Biocube Quarantine Tank = QT

Header Tank = Header Tank = HT

Process

All samples will be processed following the established Putnam Lab Titrator Protocol.

All data processed and collected on the Putnam Lab Titrator will be available on the Putnam Lab Titrator GitHub Repository.

Data for samples run on 20220118

Notes

  • Before the titration run, salinity was measured following the conductivity calibration protocol.
  • CRM was a little off but was getting older, still in acceptable range.

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20220118 2203.673 2224.47 -0.934919329 180 CRM opened 20210720

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2001.90183665432 60.433 35
QT1 2004.95831881696 60.102 34.24
QT2 2011.45544773809 59.852 34.24
BT1 1789.09768454304 60.367 33.54
BT2 1788.04911162467 59.866 33.54
BT3 1787.83880291917 59.91 33.54

Data for samples run on 20220127

Notes

  • Before the titration run, salinity was measured following the conductivity calibration protocol.
  • New CRM bottle opened and acid titrant changed 20220127 BATCH # A22
  • Samples for header tank taken at 12:00 pm for Trident curve.

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20220127 2231.417532 2224.47 0.312323022 180 CRM opened 20220127

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 1930.418106685 59.896 35
HT1 2772.05903590329 59.672 35.53
HT2 2770.66253255787 59.538 35.53
BT1 2771.70551884888 59.849 35.48
BT2 2772.28127754782 59.528 35.48
BT3 2770.82762300055 59.763 35.48
QT1 2828.65009914292 60.09 35.36
QT2 2829.83274101129 60.172 35.36

Data for samples run on 20220131

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20220131 2232.37 2224.47 0.355140775 180 CRM opened 20220127

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2593.49792303463 59.912 35
HT1 2709.36597430246 60.393 34.55
HT2 2710.26314243623 59.841 34.55
BT1 2708.42360442668 60.373 34.28
BT2 2708.51057799141 59.978 34.28
BT3 2706.96285925233 60.237 34.28
QT1 2813.16567425875 60.482 34.01
QT2 2814.39469561361 59.512 34.01

Data for samples run on 20220201

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20220201 2228.256467 2224.47 0.170218852 180 CRM opened 20220127

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2636.87337125827 59.672 35
HT1 2698.76846972992 60.355 35.71
HT2 2698.66660047764 60.217 35.71
BT1 2703.99251926485 59.711 35.4
BT2 2702.33173301221 59.963 35.4
BT3 2699.67280576546 60.462 35.4
QT1 2817.80180338956 59.907 35.33
QT2 2818.26455996811 59.878 35.33

Data for samples run on 20220203

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20220203 2226.157639 2224.47 0.075867023 180 CRM opened 20220127

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2652.01868843782 60.252 35
HT1 2686.50589379584 59.991 35.61
HT2 2683.72029072224 60.372 35.61
BT1 2688.06190840313 60.247 35.2
BT2 2690.26497313937 60.139 35.2
BT3 2688.90731610684 60.408 35.2
QT1 2823.76279986172 60.342 34.84
QT2 2818.53715332585 59.842 34.84

Our undergraduate, Kristen, took over titration responsibilities between 20220209 till 20220518

Data for samples run on 20220608

Notes

  • Before the titration run, salinity was measured following the conductivity calibration protocol.
  • When I looked back at the CRM accuracy data ran in the past three weeks, I noticed that the values were <1% off in terms of accuracy after troubleshooting and checking that there were no bubbles in the acid line and the pH values were good. I also opened a new CRM bottle because I thought maybe the CRM had just sat too long. Once I noticed that the second CRM I ran was still off, I knew that there was probably some contamination with the acid titrant. I then swithced out the acid titrant with a new bottle of the same batch number (A22) and ran another round of CRMs to find that that was the issue. I think that the issue was we use the purge HCl cup underneath the stirrer when the titrator is not in use some build-up of acid precipitate occurs in the cup. Moving forward, when you do the acid purge we need to make sure to first clean this cup with DI water into the waste containers because I think what was happening was the purged HCl we normally put back into the bottle was mixing with this precipitate causing contamination in the acid titrant.
  • I also trained Lauren on the titrator today and she will be taking over the CBLS titrations from now on.

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20220608 2228.8510392502 2224.47 0.196947554 180 CRM opened 20220427

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2002.77352735714 59.812 35
HT1 1736.27119513021 60.086 36.92
HT2 1735.46572189237 60.32 36.92
BT1 1738.99063457413 59.802 36.93
BT2 1735.76800715807 59.786 36.93
BT3 1739.75677216614 60.267 36.93
QT1 2485.31826306165 59.888 40.43
QT2 2487.98454750446 59.786 40.43
Written on January 31, 2022