Tracking Total Alkalinty in the Coral Polyp Bailout Tank System

Weekly Titrations to Track Total Alkalinity in the Coral Polyp Bailout Tank System

Goal

Conduct weekly titrations to track the total alkalinity of the polyp bailout tank system located on the bottom floor of the Center for Biotechnology and Life Sciences at URI. Hollie and Hannah are conducting an experiment on the dynamics of coral polyp bailout that requires stable tank conditions.

Process

Each week, triplicate water samples will be collected from the tank system and brought back to lab for titration runs.

All samples will be processed following the established Putnam Lab Titrator Protocol.

All data processed and collected on the Putnam Lab Titrator will be available on the Putnam Lab Titrator GitHub Repository.

Data for triplicate samples run on 20210328

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210328 2226.220045 2224.47 0.078672455 180 CRM opened 20210314
20210328 2224.425627 2224.47 -0.001994779 180 CRM opened 20210314
20210328 2224.201971 2224.47 -0.012049131 180 CRM opened 20210314
20210328 2225.549637 2224.47 0.04853455 180 CRM opened 20210314
20210328 2228.292488 2224.47 0.171838126 180 CRM opened 20210314

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2785.88960074103 59.833 35
TANK1 2032.99636827027 60.236 32.86
TANK2 2025.35809989408 59.601 32.86
TANK3 2026.8978478435 60.205 32.86

Unable to run titrations on 20210404 due to pH probe issues

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210404 2272.887 2224.47 2.176563406 180 CRM opened 20210404

Unable to run titrations on 20210411 due to pH probe issues

New pH probe arrived 20210413 and was tested, titrator good to use

Data for triplicate samples run on 20210418

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210418 2218.256 2224.47 -0.27934744 180 CRM opened 20210418

Total Alkalinity Output

SampleID TA Mass Salinity
junk1 2051.39244714175 59.952 35
tank1 2046.22678935555 60.234 35.58
tank2 2043.87189424588 60.347 35.58
tank3 2041.76501819174 59.78 35.58

Data for triplicate samples run on 20210428

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210428 2223.783 2224.47 -0.030883761 180 CRM opened 20210418

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2052.515651 60.179 35
TANK1 2020.933775 60.042 33.26
TANK2 2015.34837 59.839 33.26
TANK3 2010.672587 59.785 33.26

Data for triplicate samples run on 20210505

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210505 2229.088 2224.47 0.2076 180 CRM opened 20210418

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2027.23997432332 59.999 35
TANK1 1989.13570487248 60.341 33.93
TANK2 1988.33638752862 60.22 33.93
TANK3 1992.54820128628 60.278 33.93

Data for triplicate samples run on 20210526

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210526 2223.47 2224.47 -0.044954528 180 CRM opened 20210526

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2046.27262934681 60.469 35
TANK1 2041.88469882272 60.165 32.21
TANK2 2042.56549084997 59.926 32.21
TANK3 2037.4502555346 59.838 32.21

Data for triplicate samples run on 20210603

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210603 2228.967 2224.47 0.202149 180 CRM opened 20210526

Total Alkalinity Output

SampleID TA Mass Salinity
TANK1 2066.79468144144 60.3 32.14
TANK2 2070.19831122349 60.183 32.14
TANK3 2069.37369394528 59.66 32.14

Data for triplicate samples run on 20210610

Notes

  • Added DT1000 temperature probe to titrator, was tested and now taking continuous temperature measurements during titrations.
  • Before the titration run, salinity was measured following the conductivity calibration protocol.

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210610 2229.315 2224.47 0.217804691 180 CRM opened 20210526

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2074.10544477908 59.34 35
TANK1 2070.03617533076 60.017 32.99
TANK2 2072.43783887673 60.449 32.99
TANK3 2070.84952930117 59.978 32.99

Data for triplicate samples run on 20210615

Notes

  • Added DT1000 temperature probe to titrator, was tested and now taking continuous temperature measurements during titrations.
  • Before the titration run, salinity was measured following the conductivity calibration protocol.

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210615 2228.722 2224.47 0.191146655 180 CRM opened 20210526

Total Alkalinity Output

SampleID TA Mass Salinity
JUNK1 2157.0790843601 59.992 35
TANK1 2154.3808303586 60.409 33.36
TANK2 2153.98057496642 60.416 33.36
TANK3 2155.33486336584 60.433 33.36

Data for triplicate samples run on 20210624

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210624 2228.452 2224.47 0.179008932 180 CRM opened 20210526

Total Alkalinity Output

SampleID TA Mass Salinity  
TANK1 2256.14833246931 60.263 34.9 7
TANK2 2256.10907414494 60.201 34.9 7
TANK3 2256.44935146691 60.413 34.9 7

Data for triplicate samples run on 20210706

Notes

Data

CRM Accuracy Data

Date CRM value Batch value % off Batch # Notes
20210706 2230.091863 2224.47 0.25272822 180 CRM opened 20210526

Total Alkalinity Output

SampleID TA Mass Salinity
TANK1 2420.20767910642 60.463 34.77
TANK2 2424.8772397006 60.411 34.77
TANK3 2420.54981744246 59.979 34.77
Written on March 28, 2021