March 2024 Goals

March 2024 goals and daily notebook entries.

Goals

Writing and manuscripts

  1. Submit molecular underpinnings (Ch.1)
  2. A.pul gametogenesis final figures, results sections for histology analysis
  3. Marine heatwaves manuscript figures
  4. POC species identification E5

Sample processing

  1. Histological image analysis (abundance and size)
  2. Library prep for heatwave WGBS samples (12)

Data analysis

  1. BS-SNPer, filter SNPs from WGBS data
  2. Work on DEG analysis for heatwave after mapping sequences to de novo transcriptome
  3. Histological image/gonad analyses

Daily log

March 1

  • Traveling

March 4

  • Traveling

March 5

  • Traveling

March 6

  • TA 11-11:50am
  • UC Merced interview
  • +5 TA statistics work
  • CBLS GitHub fixing issue
  • Lab meeting
  • Reworked SMART goals
  • Wrote student statement for summer stipend support

March 7

  • Molec underpinnings introduction edits
  • Interview prep
  • POC files upload to Unity
  • BS-SNPer 10x file download
  • Histology image analysis

March 8

  • Austin +5 TA meeting
  • TA 11-11:50
  • E5 molecular meeting
  • Serenas defense
  • UNCW interview
  • Molec underpinnings introduction edits
  • Histology image analysis

March 11

  • Finished molec underpinnings introduction edits, sent to Hollie
  • Applied for Summer Stipend Support through graduate school
  • Histology image analysis
  • Aquarium daily tasks, calibrated orion sal probe, uploaded measurements, added TA data to CBLS wetlab
  • BS-SNPer meeting with Kevin and Emma, made GFF motif with CG sites

March 12

  • Vis-a-thon planning meeting
  • Writing/ journal club meeting
  • Feedback and edits on Ch.2 manuscript introduction
  • Sent Moorea package with supplies to Jordan in California
  • TA grading
  • Histology image analysis

March 13

  • Histology image analysis
  • Lab meeting 12-1:30pm
  • A.pul gametogenesis introduction edits
  • Knauss notification as RI finalist!

March 14

  • Writing retreat!
  • Wrote and edited three into paragraphs for A.pul gametogenesis manuscript
  • Made manuscript draft for SeaLifeBase citation analysis for +5 TA

March 15

  • +5 TA meeting
  • Citation analysis work
  • Histological image analysis
  • Edits to C. virginica Methylation and Gene Expression manuscript

March 16

  • Manuscript edits for Kevins perspective piece

March 17

  • Manuscript edits for Kevins perspective piece

March 18

  • Histological image analysis
  • TA 11-11:50, led a lecture class myself!
  • TA grading

March 19

  • Histological image analysis
  • Putnam writing meeting 11-12:30
  • SNP-WGBS meeting 3:30-4:30pm
  • Filtered SNPs from data, re-analyzed WGBS data
  • UC Merced second interview

March 20

  • Histological image analysis
  • TA 11-11:50
  • Putnam lab meeting 12-1:30pm
  • Aquarium duties and titrations
  • Vis-a-thon planning 4:30-5:30
  • Added summary for individual meetings with Hollie

March 21

  • TA office hours 9-12pm
  • +5 sealifebase manuuscript and kelp timeseries data analysis
  • RISD for vis-a-thon planning and making installation

March 22

  • +5 TA meeting 9:30-10:30
  • +5 sealifebase mauuscript and kelp timeseries data analysis
  • TA 11-12pm
  • Molecular mechanisms meeting 12-1pm
  • Re-analyzed WGBS data from filtered SNPs, wrote methods section for molec manuscript for BS-SNPer
  • POC histone and POR H2 prep, located samples, prepped protocol

March 23

  • RISD Vis-a-thon prep all day

March 24

  • RISD Vis-a-thon prep all day and showcase :)

March 25

  • Prep for 15 POC samples, ran PCR
  • Histology image analysis
  • TA 11-12pm
  • TA meeting 12-1pm
  • Ran post-PCR gel for POC samples
  • RFLP prep and completion for POC samples

March 26

  • Prep for 15 POC samples, negative and positive controls ran PCR
  • Histology image analysis
  • Ran post-PCR gel for POC samples, positive control did not cut
  • RFLP prep and completion for POC samples gel and QC round two
  • TA test corrections 14:00-16:00
  • POR H2 PCR and QC with negative and two positive controls round 1
  • POR H2 PCR with dilutions and negative and two positive controls round 2

March 27

  • BIO 101 TA 11-12
  • Histological image analysis
  • POR H2 PCR results, move forward with 837 and 439 samples
  • Nanodrop POR samples
  • Prepped POR samples for sequencing
  • Edited markdown for protocols used
  • BIO 101 test corrections 13:30-14:30
  • Hollie meeting 14:30-16:00
  • Post PCR RFLP

March 28

  • Submitted POR samples for sequencing 10am
  • POC PCR and RFLP round 3

March 29

  • TA meeting 9:30-10:30am
  • BIO 101 TA 11-12pm
  • POC PCR, post-PCR, and RFLP round 4, new, originally needed primers
  • Histology image analysis
  • BIO 101 TA grading
  • Cleaned plugs and crates for BT 3/4, other aquarium duties
  • Asked Oliver and James to put in work order for wetlab lights

March 31

  • TA methods and results sections for SeaLifeBase manuscript
  • TA Kelp Monitoring database updates
  • Hollie meeting prep, update dissertation benchmarks and timelines
  • Ch1 results and discussion updates after BS-SNPer
  • A.pul histology figure with data processed thus far
Written on March 1, 2024