May 2024 goals and daily notebook entries.

Goals

Writing and manuscripts

1. Submit molecular underpinnings (Ch.1)
2. A.pul gametogenesis final figures, results sections for histology analysis
3. Marine heatwaves manuscript figures

Sample processing

1. Histological image analysis (abundance and size)
2. Library prep for heatwave WGBS samples (12)

Data analysis

1. Work on DEG analysis for heatwave after mapping sequences to de novo transcriptome
2. Histological image/gonad analyses
3. Add biomass data for heatwave and gametogenesis

Daily log

May 1

• Finished condensing A. pulchra decalcified samples in flammable fridge B (August - November 2022)
• Edits to introduction and methods sections for ch1
• Histology analysis
• Putnam Lab meeting 12-1:30pm

May 2

• Edits to introduction and methods sections for ch1
• Ch2 figure 1 and 2 of experimental design
• Maggies defense
• Histology analysis literature review

May 3

• Added inventory for H walk in freezer to master cold storage list
• +5 TA work, statistical analysis and data organization
• BIO 101 TA final 11:30-1:30pm
• BIO 101 TA grading

May 6

• Started workflow for transcriptome filtering
• BIO 101 TA grading
• Final Ch1 edits through intro and methods section
• Histology analysis

May 7

• Histological analysis
• Update figure 1 and 2 for ch2
• Ch2 writing for methods

May 8

• Histological analysis
• Update figure 1 and 2 for ch2
• Aquarium tasks
• Putnam lab meeting 12-1:30pm, freezer organization

May 9

• Histological analysis
• Update figure 1 and 2 for ch2
• Literature review for ch2 analysis
• Data entry for biomass for ch2 and 3

May 10

• Out of town, vermont for rugby

May 13

• Histological analysis
• Update figure 1 and 2 for ch2
• Literature review for ch2 analysis
• Data entry for biomass for ch2 and 3

May 14

• Histological analysis
• Update figure 1 and 2 for ch2
• Literature review for ch2 analysis
• Data entry for biomass for ch2 and 3
• Ariana meeting molecular underpinnings figure updates

May 15

• Titrations and aquarium duties, adjusted dosing
• Finished emptying bottle waste for E5
• Putnam lab meeting 12-1:30 pm
• UNCW email and financial meeting with Hollie 1:45-2:15pm
• Histology analysis
• Literature review for ch2 variance partitioning
• Entering in biomass data for ch2 and ch3

May 16

• Histology analysis
• +5 TA computer and statistical work
• Entering in biomass data for ch2 and ch3

May 17

• Histology analysis
• Entering in biomass data for ch2 and ch3
• E5 molecular meeting
• Austin meeting, coding and working for kelp timeseries manuscript, planning for summer

May 20

• Histology analysis
• Nyssa meeting
• Updated ch2 biomass figures for Jan-March

May 21

• Ch3 updated introduction and methods, outlined results section, updated experimental design figure
• Histology analysis
• Entered biomass data for more months Ch2

May 22

• Continued working on Ch3 updates
• Hollie meeting on ch3 catch up and outlines, experimental design
• LTER committee meeting
• Updated Ch3 experimental design figure

May 23

• Continued working on Ch3 updates
• Updated Ch3 experimental design figure
• Austin meeting
• Data cleaning for kelp timeseries project
• Data analysis for kelp timeseries project

May 24

• Finished Ch3 updates for experimental design figure
• Updated methods and results section Ch3
• Austin meeting, kelp timeseries analysis updates
• Ran BUSCO and other statistics (N50, etc) on reference transcriptome assemblies
• Traveling

May 29

• Finished Ch3 updates for experimental design figure
• Addressed comments and issues for ch1
• Updated methods section for ch3
• Kelp timeseries data analysis and figure generation

May 30

• Addressed comments and issues for ch1 manuscript
• Histological analyses
• Results section ch3
• Prepared corals and dewer shipment for Barott lab

May 31

• Addressed comments and issues for ch1 manuscript
• Histological analyses
• Results section ch3
• Biomass data entry for ch3, finish publishable figures